Diego Huet, Ph.D.

Pharmaceutical and Biomedical Sciences
Assistant Professor

Professional Website

Pharmaceutical and Biomedical Sciences

Education

2009–2013    Ph.D. in Molecular Parasitology. Advisor: Philippe Bastin
Pasteur Institute and Université Pierre et Marie Curie, Paris, France

2007–2009    Master of Science in Molecular and Cell Biology, Magna Cum Laude
Université Pierre et Marie Curie, Paris, France

2004–2007    Bachelor of Science in Cellular Biology
Université Montpellier 2, Montpellier, France

Honors, Awards, and Achievements

2018    NIH K99/R00 Pathway to Independence Award

2018    Whitehead Institute Director’s Fellowship Allowance

2016    Whitehead Postdoctoral Association Educational Award

2016    Molecular, Cellular and Immunoparasitology Scientific Award at the 27th Annual Molecular Parasitology Meeting, Woods Hole, MA

2015    Molecular, Cellular and Immunoparasitology Scientific Award at the 26th Annual Molecular Parasitology Meeting, Woods Hole, MA

2012    FRM fellowship (French Foundation for Medical Research)

2009   MRT fellowship (French National Ministry for Research and Technology)

Research Interests

Dr. Huet’s current research focuses on the unusual aspects of apicomplexan biology. This group of protozoan pathogens cause morbidity, mortality and substantial economic loss. Notably, they contribute to more than half a million annual deaths due to malaria. The apicomplexan Toxoplasma gondii infects an estimated 25% of the world’s population, making it one of the most ubiquitous human parasites, and it is a leading cause of death from foodborne illness in the United States.

Selected Publications

 

  1. Huet, D., Rajendran, E., van Dooren, G. G., & Lourido, S. (2018). Identification of cryptic subunits from an apicomplexan ATP synthase. eLife, 7, 125. http://doi.org/10.7554/eLife.38097
  2. Sidik, S. M., Huet, D., & Lourido, S. (2018). CRISPR-Cas9-based genome-wide screening of
    Toxoplasma gondii. Nature Protocols, 13(1), 307–323. http://doi.org/10.1038/nprot.2017.131
  3. Sidik, M.*, Huet, D.*, Ganesan S.M., Huynh M.H., Wang T., Nasamu A.S., Thiru P., Saeij J.P.J., Carruthers V.B., Niles J.C., & Lourido, S. (2016). A genome-wide CRISPR screen in Toxoplasma identifies essential apicomplexan genes. Cell,166(6):1–26. http://doi.org/10.1016/j.cell.2016.08.019 (*equal contribution)
  4. Huet, D., Blisnick, , Perrot, S., & Bastin, P. (2014). The GTPase IFT27 is involved in both anterograde and retrograde intraflagellar transport. eLife, 3(0), e02419–e02419. http://doi.org/10.7554/eLife.02419.024
  5. Rotureau, , Ooi, C.P., Huet, D., Perrot, S., & Bastin, P. (2014). Forward motility is essential for trypanosome infection in the tsetse fly. Cellular Microbiology, 16(3), 425–433. http://doi.org/10.1111/cmi.12230
  6. Subota, I., Julkowska, D., Vincensini, L., Reeg, N., Buisson, J., Blisnick, T., Huet, D. Perrot, S., Santi-Rocca, J., Duchateau, M., Hourdel, V., Rousselle, J. C., Cayet, N., Namane, A., Chamot- Rooke, J., & Bastin, P. (2014). Proteomic analysis of intact flagella of procyclic Trypanosoma brucei cells identifies novel flagellar proteins with unique sub-localization and dynamics. Molecular & Cellular Proteomics, 13(7), 1769–1786. http://doi.org/10.1074/mcp.M113.033357